Coding

Part:BBa_K1692027

Designed by: Charles Calvet, Thai Nguyen   Group: iGEM15_Stanford-Brown   (2015-09-14)

CIPA-MSA

Overview

We used the cellulose binding domain cellulosomal-scaffolding protein A (cipA) of Clostridium thermocellum for construction of our cipA-mSA construct (BBa_K1692035) and our spore coat construct (BBa_K1692028). The cipA used is modified to get rid of the illegal ecoRI site found in the 2014 Imperial College IGEM team construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None